You'll love the tour, we just got back a few weeks ago. There is so much to seeing Rome.
You'll love the Borghese, such a awesome intimate experience with incredible art and if you head upstairs first, then downstairs; you'll be opposite the crowd. Check youtube for art historians interpreting the meaning behind the Bernini statues b4 you go. And don't google map "Villa Borghese" google map "Galleria Borghese" to find it. Don't be late and give yourself a good hour to metro then walk there.
Walk around the corner if you see the Aventine keyhole, to see the Bocca Verrata. You're a block or 2 away! Palatine hill, The Baths, the Crypts, Appian Way and Capitoline are Roman sites you may want to target. We did the Bernini/Michaelangelo hunt in the many churches and Plazzas. You'll pass random old Roman ruins as you walk around, some have descriptions some don't.
The Vatican tour is abbreviated, TELL your guide if you want to see the Raphael Rooms, they were not on our Vatican Tour, same with the Forum. Our guide sat in the shade and told stories, we didn't really see much of the forum. Don't hesitate to peel off to see what you want. Have a plan, Rome is big and to see a lot of it you need a plan or more likely an idea that may not be achieved as time always runs out.
We did an Eatwith.com dining experience in Trastervere. Barbara and Ferdinand. awesome experience and incredible food. A truly local dining experience. One of the highlights of the tour for us.
Loved Volterra. Get lost in the quaint streets and alleys. Ask the guide to describe the Etruscan Museum in detail before she bails. She will bring you in tell a brief story and she is gone. you are free too linger but walk upstairs to see the preserved tomb. The wine tasting was awesome. we bought a couple of bottles to enjoy later in the tour. The country side is beautiful as you drive to Volterra.
If you love seafood; as soon as you arrive in Monterossa, get a reservation at the Belvedere and get the Amphora!! Its a seafood ciopinni like meal served in an earthenware container at your table. Calamari, octopus, mussels, shrimp, fish and lobster in a tomato based broth. Great presentation for a great meal. Get noodle legs and do a challenging hike between hill towns. Do the boat ride organized by your guide. We saw the sun setting over the Mediterranean on our ride. Have a Spritz and swim in the ocean although it may be cold there in March and I hope things are open, as the Cinque Terre tends to sleep in the winter/off season.
In Florence have the steak! Spend some time across the Arno River. Extend your time in the Uffizi and head to the basement to see more incredible art. Get noodle legs and a timed entry to climb the Duomo for the incredible views and you'll feel every one of the 462 steps!! Gallileo museum is also interesting if you're into science. Our hotel had a Tower bar and we had a spritz as we watched the sun set and the lights of Florence come alive.
Enjoy your trip and don't stress out if you don't see all on your list! I didn't either!. Oh well, looks like we gotta go back :)