Yes, there are a number of wineries that do wine tastings. The trick is to locate ones fairly close to one another.
There are several in the general vicinity of Pinhao and I’m guessing the same is true for Régua. If you Google Wine Route Portugal you will find a number of websites that would help.
You would need a car even if you take the train from Porto up to the valley as the wineries are not all grouped together.
Also, the Port lodges in Gaia, across the river from Porto, have a few places that also have wine. I’m not a port fan but had a great afternoon doing tastings of regular wine in Gaia.