Rome Rick’s Audio Tours

I would eliminate Ostia Antica, even though it was one of the highlights of our Rome trip last October. Depending where you're staying, it's a subway ride and a train ride from Rome proper. If you had more time, I'd say don't miss it, but the other sites are more accessible.

Our train from Florence is going to Fiumicino where we are staying until the following midday flight home. Would that make it more accessible?

Our train from Florence is going to Fiumicino where we are staying until the following midday flight home.

Please provide a day by day schedule - are you intending to do the four tours on the same day after you arrive from Florence?

You could take a bus or taxi from FCO to Ostia Antica.

On our second day we will do the four audio tours indicated above. Our access to Vatican Museum and Sistine Chapel is at 3.

The OA is the day before our flight back home. We are staying at hotel Tiber that day and leave in the noon for USA. If it’s too much, it will have to wait a few more years.

Pantheon, Jewish Ghetto, Colosseum, Forum

You can do these in a day; the order may depend on what time slot you're able to land for the Colosseum. i'm assuming you're planning to see the inside, or are you just doing a walk-by? I see that you've included the Forum but not the Palatine? Your Colosseum ticket, assuming you're going inside, includes that part as well - you'd visit it at the same time as the Forum as they're both inside a shared security enclosure - and it's certainly interesting enough to add, IMHO.