I believe someone reported on this forum that the Fondaco dei Tedeschi viewing terrace in Venice is closing soon because the shopping center shuttering. It won't hurt to check, though. I wouldn't count on being able to get an online entry appointment for San Marco after you arrive in Venice, though I suppose it's possible you'll be able to do that in the Feb-Mar timeframe. It would be better to take care of that ahead of time so you know you won't have to stand outdoors in Venice in the winter, when the weather might be rather raw. You should be able to get tickets for most of the other sights in Venice on the spot in the winter. The Secret Itineraries Tour at the Doge's Palace is popular on this forum, and it does sell out in advance at least part of the year. I don't know what the situation is like in the winter. Individual vaporetto tickets are a painfully expensive at 9.50 euros per ride, so the advice to get a suitable multi-day pass is excellent unless you are confident you will move around almost entirely on foot. Here are the prices for the passes:
24 hours: 25 euros
48 hours: 35 euros
72 hours: 45 euros
168 hours (7 days): 65 euros.
Even with a pass, you must tap the validator before boarding the vaporetto for each ride.
If you want to see the Sistine Chapel in Rome, you need tickets to the Vatican Museums. Those are likely to sell out ahead of time. The Vatican sells plain-vanilla entry tickets (and you can rent an audio guide if you like, or listen to Rick's guide for free), or you can book a tour conducted by the Vatican or by a commercial tour company. The Vatican's tours are cheaper. Some tour companies offer combination tours of the Vatican Museums plus St. Peter's Basilica. The advantage of those is that the tour groups usually are allowed to use a connecting door between the Sistine Chapel and the Basilica. Without that provision, visitors who want to see both buildings must walk some distance from the Sistine Chapel back to the exit of the Museums, walk outdoors around the outside of the building, and stand in what is likely to be a very, very long security line at the Basilica. The Museums and the Basilica will probably be a bit less busy in Feb/Mar than later in the year, but I was in Rome during Mar 2023, and the sections of the Museums along the most direct path to the Sistine Chapel were unpleasantly crowded. And 2025 is a Holy Year, expected to send more visitors to Italy and especially to Rome.
You can buy Vatican Museum tickets here: https://tickets.museivaticani.va/home/fromtag/2/1740373200000/Biglietti-Musei. It appears the Vatican's tours aren't yet on sale for your dates; they should be available on that same website if you want a tour and are comfortable waiting to make the purchase.
There is no entry fee for St. Peter's so there's no way to buy a ticket ahead of time to circumvent the long security line--unless you take a commercial combination tour, as mentioned above (which will probably cost in excess of 100 euros per person).
Online tickets for the Colosseum usually sell out earlier than tickets for the Vatican Museums; I don't know how early that will happen in Feb/Mar. They changed the ticketing procedures about 11 months ago, to try to keep tour companies from grabbing so many of the tickets. Still, if you want to see the Colosseum, you should check immediately for information here on sales procedures. It would be prudent to buy Colosseum tickets the day they go on sale for your desired date, and it's possible you'll need to get up in the middle of the night to grab them as they are put on sale. (But only if you feel you have to go inside the Colosseum; the exterior is free to view.)
Another sight in Rome for which tickets should be purchased ahead of time by those interested in seeing it is the Borghese Gallery.