Happy Easter (or Passover, for our Jewish friends) to you as well!
Wednesday May 8th Villa Borghese gardens and Galleria Gorghese, Santa
Maria della Concezione crypt, 1:15 Colosseum, Roman Forum and Palatine
Hill Tour, Baths of caracalla, Appian Way
Ooof! All of these are so interesting but you're simply not going to be able to do them all in one day. The Colosseum, Roman Forum and Palatine Hill Tour alone will be wearing, and Villa Borghese (the gardens) covers a lot of ground so with just these + Galleria Borghese, you've already got a long day on your feet. I'd consider removing the Baths and Appian as I can't see you having enough steam left to fully explore and appreciate them.
We're stout walkers/hikers and have done some long days in Rome (e.g hiking the Appia from Casal Rotundo back into the city, hitting Circus of Maxentius, San Sebastiano catacombs, Capo di Bove, Domine Quo Vadis, Baths of Caracalla, San Giovanni in Laterano, Santo Stefano Rotondo and San Clemente) and I know we'd struggle to manage your itinerary, not that I'd even try to.