Jules, it's difficult to tell someone else what they should keep and what they should axe from a wish list so all I can give you is my own 2 cents? I don't think you can stuff all of this into 2 days + part of another but you can probably do most of it. We had almost 5 full days and had to be selective but we also may have spent more time on some of the attractions than you plan to, and we did some which are not on your list.
RS Renaissance Walk: I don't think this needs to be an individual thing, if that makes sense? You will be either visiting a lot of what's on the map or passing by from one attraction to another so just mark those info on those attractions or route as being included in the walk material.
Duomo, museum, baptistry, dome, campanile: we skipped the dome and the museum was closed for renovation so we didn't get to see it but definitely would have otherwise. Your ticket will cover ALL parts of the complex and is good for 72 hours from first entry so is a very good deal but you would have to make a prior reservation for the dome...which could end up being at an inopportune time. As you're already going to need to make timed-entry reservations for the Uffizi and probably the Accademia, I don't know if I'd want to be to nailed yet another entry. Also, the ticket doesn't provide priority entry to the church - everyone stands in the same entry queue - and that can eat time. Personally? We found the exterior of the duomo much more impressive than the interior - and especially when lit up at night - and could have given it a skip if time was of the essence.
Pitti, Boboli (and/or Bardini Gardens) Ponte Vecchio, Oltrarno District, Piazzale Michelangelo: all of this should be combined, and it will easily eat an entire day. Use Ponte Vecchio to get across the river, The Pitti is immense - Palatine Gallery and Royal Apartments: 28 and 14 rooms, respectively - as are the gardens, which are long on clipped shrubs/ hedges, trees, lawns and water features and short on flowers. Quite a few posters have preferred the less sprawling, more flower-y Bardini Gardens nearby; I believe your ticket Boboli ticket covers that as well. Anyway, end the day at Piazzella Michelangelo for the view: that's what we chose for our bird's-eye spot versus towers or the cupola: less confining. If you get there early enough, climb up a little further to lovely San Miniato al Monte: highly recommended!
https://www.visitflorence.com/florence-churches/san-miniato.html
A note on Ponte Vecchio? Our least favorite place to appreciate the bridge was ON the thing. It's more interesting seen from the outside and without the mob.
Medici Chapels: the thing we wish we would have skipped. It's an ostentatious thing for sure but underwhelming compared to many other attractions we chose to see in Florence, and has my least faves of Michelangelo's carvings.
We didn't do Palazzo Vecchio but Piazza della Signoria can be walked at any time - it's especially atmospheric at night - and the small sculpture collection at open-air Loggia dei Lanzi is free and well worth a look:
https://www.visitflorence.com/florence-monuments/piazza-della-signoria.html
Combine a walk-through of Plaza S.S. Annunziata with the Accademia as it's nearby. I'd love to tell you to see Museo San Marco as it's right there too and we really enjoyed that one - I'd sure choose it over the Medici Chapels - but I know you can't do it all....