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Best town in Tuscany Area to base out of

We will be in Rome through 4/1/12 and plan to either take a train or bus to towns in the Tuscany area. Which town would be the best to serve as a base to travel to surrounding areas. We would prefer to use public transportation rather than drive. Would enjoy biking in the areas as well.

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Florence or Siena will likely give you the best options for access. As I sure you know, though, Tuscany is really best traveled by car. I've done is by train and bus (on my first trip) and it was great, until I went back and got a car. Then, no comparison. I'd consider picking up a car for a few days while there.