Is travel a problem on All Saints Day( Nov.1 )in Italy especially from city to city ?by bus
This kind of question, requiring very specific familiarity with the exact areas, with bus schedules, and on the holiday in question--if it can be answered at all--is probably best posted at this website, picking one of the forums that matches your itinerary
The trains should run fine but you can bet that the inter-city buses will be on what is known as "Festival" schedule. This usually indicates a reduced schedule. Where are you intending to travel? Italy has a very large quantity of private bus systems and they all have their different schedules. Tuscany has about a dozen bus companies alone.