Alsace region for 5 nights

When we visited for 6 nights in April, we stayed in Colmar without a car, but after visiting several towns I would stay next time in Ribeauville. Big enough to be interesting in the evening with restaurants and shops, but small enough to be charming with a big pedestrian zone.

We stayed in Ribeauville several years ago and it was a great central location. Great town to explore. Lots of good restaurants and shops. Many wineries nearby.

I stayed in Eguisheim. It's very close to Colmar. Other villages are also charming and good locations.

It's best to take the train to Strasbourg and pick up a rental car there, where there are several companies. Strasbourg is worth a day - maybe return the car and store bags at the rental agency or the train station, rather than a day trip from the village where you're staying. It's about an hour's drive.

We visited many of the other towns mentioned, but stayed in Colmar based on the recommendation of a very well-traveled friend. I would do the same again if the opportunity presents. Lots to do in Colmar itself (restaurants, museums, etc.) but we spent most of the time on the Alsace wine road, using Colmar as our base. We drove from Champagne region, via Burgandy, to Alsace region, which we loved! We had no issues driving in/out of Colmar, although parking was dodgy depending on whether your hotel/inn has parking.
Upon departure, we dropped our rental car at Strasbourg. Strasbourg is worth a visit, for sure, and a transportation hub via Paris, etc.

We spent a week in Riquewihr. Although it was w/o a car, it would be a great place to stay with a car, except that you can't park right in the center. Others have suggested Ribeauville, which would also be a great choice. So would Kaysersberg.

We also stayed in Riquewihr but all the wine villages are charming. The only place we didn’t care for was Colmar.