Traveling from Prague to the Budweiser Brewery in České Budějovice

By train it takes about 2.5 hours. There are departures nearly hourly throughout the day.

Tickets online at CD eshop

been a couple of years since I did the trip but took the train and as Podroznik says takes about 2.5 hours.
Once at the station it is about a 20 minute walk to the brewery and it is set in the middle of an industrial estate, not the prettiest of locations but once in the actual brewery it is nice.you could probably get a taxi from the train station
check what time the tours are, I think it is 2.oopm or 2.30 pm for walk up tours that are done in Czech and English.the free beer samples on the tour are pretty generous, it is taken directly from the lagering tanks and is quite superb,make sure you grab an extra one to take with you whilst you visit the bottleing hall.
The town itself is worth a wander through and I think you would need at least an additional hour or so for that. the walk to the brewery took me about 20 minutes from the main town square, easy enough route on a fairly straight road.

sorry I should say I just bought my train tickets on the day I travelled at the main train station, really cheap.

I have been there last summer. It's very easy by public transportation. You can go from Prague by train or by bus. It takes about the same time. It looks that there is some construction going on railroad between Tabor and Ceske Budejovice. That means that from Tabor to C.B. you would go by bus. Check it when you are in Prague if it still the case. I would recommend Student Agency bus so called Yellow bus. It leaves from bus station "Na Knizeci" in Prague and goes to Cesky Krumlov. It stops at bus station in Ceske Budejovice which is next to the train station. From there take trolleybus #1, 3 or 9 to Senovazne namesti,posta; change to trolleybus #2, get off at Budvar stop and you are there. From the train station to Budvar stop it takes about 11 minutes. Cost 13 czk - quite cheaper than taxi and quite faster and less tiring than walk. You will buy trolleybus ticket at the tobacco or newspaper stand, then validate inside the trolleybus. Similar like in Prague. Some websites about brewery tour: http://visitbudvar.cz/en/prohlidky/
http://www.outsideprague.com/ceske_budejovice/budvar_budweiser_brewery_tours.html
And you can kill two birds with one stone and see the town, too. Pretty main square and streets around.
You can check train and bus schedule at: http://www.idos.cz
I would not recommend to drive there from Prague. Not only it's much more expensive than public transport but because of zero tolerance of alcohol behind the wheel in C.R. you would not be able to taste their Budweiser which would be very regrettable because IMHO Budweiser from Budweis is much better than Budweiser from St.Louis.

Thanks very much everyone for all of your helpful responses. Na zdravi!

Ilya and Unclegus have it all covered, but I would add: get there hungry. We did the tour a month ago, and due to dicey weather, decided to eat a very early dinner at the brewery. Can report that the attached restaurant is überworthy, with friendly staff and solid, reasonably priced food. Probably going back again in a few weeks because I found it decidedly challenging to sample a half liter of each of the offerings on tap. Shame too because coming from Germany it's like a neverending "half off drafts" in the Czech Rep.

Anyways, try the duck. Menu described 1/4 duck, but I'm pretty sure (limited knowledge of waterfowl anatomy notwithstanding) that was a half a duck on the plate for ~8€. My wife claimed it was far and away the best duck she'd ever had. Granted, we're probably not talking about a sample size in the triple digits, but refer back to the opening sentence of this paragraph if my rambling commentary has induced bewilderment.

PS: when we go again, I'll inquire about annual dues for a mug locker that could be reserved for alums (PSU '91 here).

Rob D: Thanks for the restaurant review; we are planning to eat there after the brewery tour. I also like the mug locker idea! (PSU '75)